Transformation of a short-term boar semen extender into a long-term boar semen extender by using penicillamine

This study aimed to convert a short-term semen extender into long-term by adding penicillamine (PEN). Semen samples were collected from six boars, diluted at concentration 3 × 10<sup>9</sup> spermatozoa/100 ml, and divided eight groups as follows: BTS, Merck III<sup>®</sup>, Androstar<sup>®</sup> Plus, PEN 0.125 mM, 0.25 0.5 1.0 2.0 mM. All the stored 18 °C examined for progressive motility, viability, acrosome integrity, pH osmolarity on days 0, 1, 3, 5 7 after storage. The level of total anti-oxidative capability (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) malondialdehyde (MDA) also measured antioxidant activities. Fertility tests commercial pig farm was conducted. On 1 storage, no statistically significant differences in all parameters among extenders observed. day 5, with mM Plus could maintain motility reach 70.0% 76.0%, respectively (P < 0.01). 7, maintained (70.0%) integrity (85.7%) which is comparable (Androstar<sup>®</sup> Plus) For activities, T-AOC, SOD MDA varied depended concentrations . results fertility test not different groups. To conclude, BTS able transform extender.